Both readers were heated to 37ºC prior to setting up . Molecular Devices' microplate readers. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Microplate is validated by molecular devices for the FLIPR reader. 020 fmol 0. In addition, the iD5 reader measures TRF and FP and. Molecular Devices gmbH Tel. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Documentation:1. Molecular Devices' microplate readers. absorbance, fluorescence, and luminescence with the added functionality of. Microplate readers use a vertical light path so the distance of the. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Can read any standard cuvette, micro cuvette, test tube and 96-well or UV-transparent microplate in the wavelength range of 190-1000 nm. Reading Capabilities: cuvette or microplate. Molecular Devices provides. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. 2. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Multi-Mode Microplate Reader for . Place the microplate in the drawer. Average StDev (mP) 1. Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. a white 96-well half-area microplate (Corning cat. You can add your own protocols. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. both cuvette-based and microplate reader applications SpectraMax Mini Multi-Mode Microplate Reader. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. We have been using this for DNA measurements 260/280 nm, colorimetric assay measurements like MTT assays, and ELISAs. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. These robust readers have been placed in labs from. I contacted Molecular Devices and they quoted me almost $6k to come out and replace. Microplate Reader (Molecular Devices cat. Easy-to-use, intuitive, configurable microplate readers with industry-leading, SoftMax Pro microplate software solution for increased productivity. A. With various on-board features and capabilities,. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Published Dec 16, 2020 | Updated Dec 16, 2020. In addition, the iD5 reader measures TRF and FP and. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. ValitaTiter 96-well (Pack of 20). Softmax Pro GxP Software. Go to Product Details. 96-well microplate at 200 µL/well. As a result, assay flexibility is consequence-free. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Description. #N207) LAL Reagent Water (Lonza cat. For more sample throughput, both readers can be easily integrated into full roboticThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Multimode Readers Comparison Chart. 5 mm. Our microplate. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Other Molecular Devices readers with absorbance detection mode, e. 984. Step 2: Launch SoftMax Pro Software. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. 50 racks/case for 384-well (16 channel pipettor head. D. Avoid losing high signal. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. The Molecular Devices Versamax Microplate Reader INCLUDED:. Measure more accurately. automation for any lab. EMax Plus Microplate Reader, Molecular Devices,96 Well Plate, LLC (CAT#: STEM-LE-0510-LC) Cytation™ 1 Cell Imaging Multi-Mode Reader, Wavelength Range: 200 to 700 nm, BioTek Instruments (CAT#: STEM-MR-0003-LKN) Perkin Elmer EnVision 2105-0020 Multilabel Microplate Reader, Perkin Elmer (CAT#: STEM-S-0723-LGZ)The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. for ValitaTiter Plus standards read on Molecular Devices readers. Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. It features a modular design that allows users to add or change detection cartridges according to their needs. (FP) configured microplate readers. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. When reading at wavelengths. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). iD5. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. ± 0. For more sample throughput, the system can well. spectramax i3x injector cartridge, smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. It measures the optical density (OD) of samples in both 96- and 384-well. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. It also allows for the elimination of standard curves. 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. the appropriate well of the microplate. (12) Variants in ASPH cause exertional heat illness and are associated with malignant hyperthermia susceptibility Nature CommunicationsDecember 1, 2022 Yukari Endo, Linda Groom, Alper Celik, Natalia. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aThe Molecular Devices Aquamax 4000 With 96 Well Head AQUAMAX 4096-G is a quality addition to the Molecular Devices lineup. 3. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Average StDev (mP) 1. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. Reactions catalyzed by firefly and *Renilla luciferases. 00 Add to cart; Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. Learn more. 5 mm and 15. /well integration in 96-well luminescence mode and 0. Both readers were heated to 37ºC prior to setting up the assay. System setup and. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. If read values are low, try setting PMT sensitivity to High in the Settings menu of the Plate section. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 012 fmol 0. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The EMax® Plus Microplate Reader is designed to be a versatile and robust microplate reader for research laboratories. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. This page contains the. 14: Average %CV. SpectraDrop High-Throughput Screening (HTS) Kit. Molecular Devices M5 Plate Reader Plate Calibration Form . explore more. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. The system provides options for 20-, 40-,. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. Optical absorbance and fluorescence were measured on a SpectraMax M5 Multi-Mode microplate reader (Molecular Devices, San Jose, CA). Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Both COC microplates, the µCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Category 2 and 3 microplate readers use gradient heating as well. 2 nm Wavelength Bandpass 2. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. 0 OD at. Read the microplate Step 1: Place the microplate in the reader. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent:. white 96 well plate and 75. SpectraMax iD5. 030 fmol Fluorescence Polarization (FP). Triplicates of each BSA concentration were made for this assay plate. 5. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Specifications for the SpectraMax L microplate reader. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. # 651101) the iD5 reader’s filter system. Read More. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. • 0. Plate Formats: 6 to 384-well microplates. ScanLater Western Blot Detection System. SOP Title: Use and. style. #3676). Description. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. Learn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are formed in single well constructs 12. The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. 03 pM. to each well. Fluorescent DNA quantitation. Learn more about how absorbance is measured, and some key applications that utilize. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. measurements in compatible Molecular Devices® microplate readers. 0 500 1000 1500 2000 2500 3000 3500 time (sec. It includes both shaking and incubation functions, and is compatible with 96- well microplates. Omnia® Compatible Microplate Reader Documentation Version No. tecHnical SPecificationS Performance Specifications Measurement range: 400–750 nm 0–4. Click on the crosshair and move it so that it is centered over the well image. Top or bottom detection is available with a simple click of a button. ODs were measured in 96 well plates with a plate reader (Molecular Devices, SpectraMax ABS Plus) with two technical replicates of each of. in a 96-well microplate. Microplate luminescent reader assay for ERK activity. The procedure enables direct translation of any published equation. NanoRacks added it to their line of commercial . 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). Microplate Spectrophotometers. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Model:. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. accurately over a wide dynamic range. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. • SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Detection by Low noise PMT with simultaneous digital and analog modes. 2600A October 2023. Reader. The microplate washer automatically washes all wells of the assay plate, using the desired wash program to completely remove unbound reagents and eliminate background noise. F3/F5 Multi-Mode Microplate Readers. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. in space. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633. Note: SoftMax Pro Software must be installed on a computer connected to the instrument. 5. Temperature Control: Ambient +5C up to 42C. #0310-5227) 96-well cell wash head (Molecular Devices Cat. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. BSA concentration (µg/mL) Average OD. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. For more sample throughput, the system can be. Timothy Bolus, MPM, MBA, Compliance Program Manager. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. Three kits covering a wide range of dsDNA concentrations. microplates or in a cuvette at a selected. #L6876) Guanidine hydrochloride (Sigma cat. Microplate method: Pipet 150 µL aliquots of each sample, standard and blank into the designated wells of the microplate. #N207) LAL Reagent Water (Lonza cat. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ EMax Plus R2 = 0. Determine DNA concentrations precisely and . Solid black plates usually give the least fluorescent background noise, allowing better sensitivity. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Paradigm: Delta mP. Paradigm: Delta mP. 76 L x 85. Less background noise gives a better limit of detection and so a relative increase in usable signal. Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. StakMax. 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl Recommended ArticlesA method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in. ( a ) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. The SpectraMax ABS. 2 Pages. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. The device was able to achieve 3σ limits of detection of ca. Reading a Microplate . 00 Add to cart; Molecular Devices EMax Microplate Reader $ 1,295. Microplate readers can be either single-function instruments or “multimode. The plate reader optics are tunable for. 100 μL/well Steady-Luc working solution was added to the wells. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. This can increase throughput and efficiency, thus saving time and space in the lab. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Molecular Devices i3x. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Optical System: High energy xenon flash lamp. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. Fluorescence Microplate Reader Comparison Chart. microplate . Microplates . Simple, economical . DNA concentration (ng/mL) Average RFU. White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 0. Suggested plates are black walled. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Application Area: Spectrophotometer for molecular biology. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the. A single-mode plate reader is a good starting point. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. and luminescence microplate reader validation DATA SHEET. About;. 2 2601A ValitaTiter. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesThe SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . SpectraMax Mini Multi-Mode Microplate Reader Afordable multi-mode microplate reader with best-in-class data analysis software Perform your favorite applications including. 100-μL samples ranging from 1 nM to 10 μM were added to triplicate wells of a solid white 96-well microplate. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). Detergent Compatible Bradford assay. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. 747. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume. The microplate reader then generates data following a preconfigured protocol that performs the necessary calculations on the data, delivering consistent results. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. To confirm the accurate positioning of arrayed colonies, we performed an absorbance scan in a 96-well scheme with 30 × 30 scans per well for an OmniTray agar plate with 96. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 1 pM. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. 300 µL of Detergent Compatible Bradford reagent was added to each well. Use black microplates. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. " A versatile UV/VIS spectrophotometer for both 96- and 384-well microplates as well as cuvette. 96-well microplate; Immunoassay analyzer;VersaMax price. This richly featured reader gives you the speed, convenience, and economy of monochromator-based wavelength selection for visible absorbance measurements between 340 nm and 850. emax and Vmax microplate readers. Step 1: Turn on your microplate reader. Published Dec 16, 2020 | Updated Dec 16, 2020. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. Use standard plate unless you see your type listed. microplates or in a cuvette at a. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. adjust the reader. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. explore more. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. Outer wells are aligned for proper viewing with FLIPR. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. #I2511) • Assay diluent – may be cell culture medium. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. For more sample throughput, the system canRating: 3. 6:30 . No additional manuals are available. 14: Average %CV. Backed by the Molecular Devices warranty, these tips are manufactured specifically to work with the FlexStation 3 instrument under strict controls to guarantee optimal performance and quality. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Detection modalities are shown in Table 1-1. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. The EMax Plus reader measures flat and round 96-well microplates. High. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. ©2017 Molecular Devices, LLC . buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Microplate Capacity Standard 96-well microplates Incubation Temperature control up to 50ºC Temperature uniformity ± 0. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Get. Plate readers, also known as microplate readers or microplate photometers,. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. SoftMax Pro Software’s. 5°C at 37°C. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Flexibility to read a variety of plate formats. Molecular. Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Five preferred modes for diverse bioresearch applications. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to.